6 well plates (Eppendorf AG)

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Eppendorf AG 6 well plates
6 Well Plates, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 205264 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/6+well+plates/Eppendorf/pmc13627152-70-11-13
Average 99 stars, based on 205264 article reviews

6 well plates - by Bioz Stars, 2026-10

99/100 stars

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In Vitro:

Article Title: Oxidative stress and GPX2 control pancreatic vs. non-pancreatic cell fate in human endoderm
Article Snippet: To estimate the effect of selenium, sodium selenite (Sigma-Aldrich, USA) in the concentration from 1 pM to 1 nM was supplemented to the medium for 3 days starting from the 5th day of differentiation. .. For in vitro pancreatic differentiation in 2D culture, we employed a protocol established previously with modifications , . hPSCs were dissociated into single-cell suspensions using TrypLE Select (Thermo Fisher Scientific, Netherlands) and plated at a density of 2 × 10 6 cells/well in Stem Flex medium (Thermo Fisher Scientific, Netherlands) supplemented with 10 μM Y-27632 (Peprotech, USA) on Geltrex-coated 6-well plates (Eppendorf, Germany) under a 5% CO 2 atmosphere. .. On the subsequent days cells were washed in DMEM/F12 medium (Corning, USA) and the medium was changed, as follows: Basal medium: S1 – MCDB131 (Corning, USA), 1% Glutamax (Thermo Fisher Scientific, Netherlands) + 1% Pen/Strep (Thermo Fisher Scientific, Netherlands), 8.88 mM glucose (Merck Millipore, USA), 14 mM sodium bicarbonate (Merck Millipore, USA) and 0.1% BSA (Capricorn Scientific, USA).

Article Title: Oxidative stress and GPX2 control pancreatic vs. non-pancreatic cell fate in human endoderm.
Article Snippet: To estimate the effect of selenium, sodium selenite (Sigma-Aldrich, USA) in the concentration from 1 pM to 1 nM was supplemented to the medium for 3 days starting from the 5th day of differentiation. .. Pancreatic hPSC differentiation in 2D culture For in vitro pancreatic differentiation in 2D culture, we employed a protocol established previously99 with modifications 17,18. hPSCs were dissociated into single-cell suspensions using TrypLE Select (Thermo Fisher Scientific, Netherlands) and plated at a density of 2 × 106 cells/well in Stem Flex medium (Thermo Fisher Scientific, Netherlands) supplemented with 10 μM Y-27632 (Peprotech, USA) on Geltrex-coated 6-well plates (Eppendorf, Germany) under a 5% CO2 atmosphere. .. On the subsequent days cells were washed in DMEM/F12 medium (Corning, USA) and the medium was changed, as follows: Basal medium: S1 – MCDB131 (Corning, USA), 1% Glutamax (Thermo Fisher Scientific, Netherlands) + 1% Pen/Strep (Thermo Fisher Scientific, Netherlands), 8.88 mM glucose (Merck Millipore, USA), 14 mM sodium bicarbonate (Merck Millipore, USA) and 0.1% BSA (Capricorn Scientific, USA).

Single Cell:

Flow Cytometry:

Article Title: Novel Cytotoxic Pt(IV) Compounds with Improved Safety Profiles
Article Snippet: .. For the flow cytometry studies, cells were plated into 6-well plates (Eppendorf, Hamburg, Germany; HCT-116 cells, 4 × 10 5 cells in 2 mL of DMEM) and incubated for 24 h. Solutions of complexes in DMSO were prepared immediately prior to the day of the experiments. ..

Article Title: Novel Cytotoxic Pt(IV) Compounds with Improved Safety Profiles.
Article Snippet: .. For the flow cytometry studies, cells were plated into 6-well plates (Eppendorf, Hamburg, Germany; HCT-116 cells, 4 × 105 cells in 2 mL of DMEM) and incubated for 24 h. Solutions of complexes in DMSO were prepared immediately prior to the day of the experiments. ..

Incubation:

Transfection:

Article Title: In vitro analysis of the effects of intronic variants c.940+3_940+6del, c.941-3C>G, and c.2389+5G>A in the LDLR gene on pre-mRNA splicing using a minigene assay
Article Snippet: Both cell lines were cultured in DMEM/F12 medium (Servicebio, China) supplemented with 10 % fetal bovine serum (Capricorn, Germany) and 2 mM penicillin/streptomycin (Capricorn) at 37 °C in a humidified incubator with 5 % CO2. .. Cell lines were transfected with either mutant or wild-type minigenes in 6-well plates (Eppendorf, Germany) using Lipofectamine 3000 (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions. ..

Mutagenesis:

Multiple Displacement Amplification:

Article Title: Molecular Basis of Simalikalactone D Sensitivity in Triple-Negative Breast Cancer Cells
Article Snippet: .. To assess the effect of SKD on cell proliferation, MDA-MB-231, MDA-MB-468 and SUM-149 cells were seeded at a density of 4.5 × 10 4 cells/mL in clear 6-well plates (Eppendorf, Hamburg, Germany) and allowed to adhere overnight under standard cell culture conditions (37 °C, 5% CO 2 ). .. The next day, cells were treated with SKD at final concentrations of 25 nM and 100 nM for 24, 48, and 72 h. At each point, cell proliferation was assessed by detaching cells from the wells using trypsin-EDTA solution (Sigma-Aldrich, St. Louis, MO, USA), followed by neutralization with complete medium.

Article Title: Molecular Basis of Simalikalactone D Sensitivity in Triple-Negative Breast Cancer Cells
Article Snippet: .. To assess the effect of SKD on cell proliferation, MDA-MB-231, MDA-MB-468 and SUM-149 cells were seeded at a density of 4.5 × 104 cells/mL in clear 6-well plates (Eppendorf, Hamburg, Germany) and allowed to adhere overnight under standard cell culture conditions (37 ◦C, 5% CO2). .. The next day, cells were treated with SKD at final concentrations of 25 nM and 100 nM for 24, 48, and 72 h. At each point, cell proliferation was assessed by detaching cells from the wells using trypsin-EDTA solution (Sigma-Aldrich, St. Louis, MO, USA), followed by neutralization with complete medium.

Cell Culture:

Article Title: Molecular Basis of Simalikalactone D Sensitivity in Triple-Negative Breast Cancer Cells
Article Snippet: .. To assess the effect of SKD on cell proliferation, MDA-MB-231, MDA-MB-468 and SUM-149 cells were seeded at a density of 4.5 × 104 cells/mL in clear 6-well plates (Eppendorf, Hamburg, Germany) and allowed to adhere overnight under standard cell culture conditions (37 ◦C, 5% CO2). .. The next day, cells were treated with SKD at final concentrations of 25 nM and 100 nM for 24, 48, and 72 h. At each point, cell proliferation was assessed by detaching cells from the wells using trypsin-EDTA solution (Sigma-Aldrich, St. Louis, MO, USA), followed by neutralization with complete medium.

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